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Measurement of tissue impedance during electroporation
Uher, Jakub ; Novotná, Veronika (referee) ; Červinka, Dalibor (advisor)
The Bachelor's thesis is devoted to the impedance of tissue in relation to the modern medical method electroporation. The Bachelor's thesis is divided into five chapters. The first three chapters describe the behaviour of the tissue and its impedance, whether during the passage of an electrical current or a phenomenon before and after the electroporation procedure. The fourth and fifth chapters examine those phenomena in the previous chapters on measurements made on animal and plant tissue.
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System with AC generator for cell electroporation
Lukoianov, Petr ; Folprecht, Martin (referee) ; Červinka, Dalibor (advisor)
This bachelor thesis is observing a system with AC generator for cell electroporation. The first paragraph keeps an eye on description of methodology of biophysics mechanisms. In the 2nd part several kinds of devices were described. In the last paragraph you can see a non-assembled construction and operating principle of the device. Apart from that the control circuit logic explored and device manual is included.
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Controlled high voltage source
Chloupek, Jiří ; Hanák, Pavel (referee) ; Koton, Jaroslav (advisor)
The aim of this master thesis is to design a high voltage source for the generation of bipolar pulses, which are suitable for the process of reversible and irreversible electroporation. Further design is electronic control. The first part of the thesis analyzes the theoretical knowledge about the process of electroporation, the second part describes the principles of realization of such sources, the next part deals with the design of the source and its control. The penultimate part is a description of the user manual and the last part is dedicated to the measurement.
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High-voltage method for measuring the specific conductivity of tissue
Uher, Jakub ; Novotná, Veronika (referee) ; Červinka, Dalibor (advisor)
This master´s thesis is dedicated to the ever-improving medical method - electroporation. This work is divided into six chapters. The first two chapters describe what electroporation entails and how it affects the tissue, how we can evaluate it and how much influence the change in tissue temperature has on measurements. The third chapter deals with the design of coaxial arrangement of electrodes - from the initial simulation to the physical product. In the fourth chapter, the measuring workplace is shown. The penultimate chapter is described and processed by individual experiments carried out on animal tissue. The last, sixth chapter summarizes the possible inaccuracies that arise in the measurement and therefore affect the results.
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Conductive model of human tissue for electroporation purposes
Zelinková, Kateřina - Šarlota ; Cipín, Radoslav (referee) ; Novotná, Veronika (advisor)
This thesis solves the issue of the distribution of the high voltage pulse at the electroporation of the arget ablation zone. For futher examination of influence electroporation was necessary to first understand theoretically electrical charakter of cells and replace with basics eletrical circumferential elements (RLC). Based on this knowledge, theoretical prerequisites for experimental measurements were derived. Theoretical assumptions were then verified by experimental measurements. In the measurement, the assumptions found were confirmed, but significant performance losses were identified. A new catheter was designed to reduce the loss. Because the production of a new catheter is not economically disadvantageous, the properties of the new catheter were first tested using the simulation cloud environment SIMSCALE, where the heating of the heart tissue after the application of the electroporation pulse was monitored. Furthermore, the electroporation process was simulated in the FEMM program, where emphasis was placed on the propagation of cardiac tissue tension.
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Controlled source of pulsed electric field
Burian, Josef ; Kubánek, David (referee) ; Koton, Jaroslav (advisor)
The aim of this master thesis is to design a power source for generating pulsed electric field for the needs of the technological process of electroporation of fruit musts and mashes. To design further the network of switched capacitors and inductors based on the required pulse and to design and implement the basic control unit together with the switching transistors. The thesis will include calculations and simulations used in the draft, also design solutions and measured values. This thesis is divided into several basic parts. In the first part there is discussed in detail the theoretical knowledge of electroporation and the desired characteristics of generated pulses are chosen according to this knowledge. Each part of electroporation workplace is described in the second part of this thesis, beginning from the source through the control system to the electrode chamber. For each of these parts are given different possible alternatives. In the next chapter is already proceeded to the design of the source. There are listed required parameters of the pulses and according to them calculations and the design are gradually carried out. Another chapter deals with the simulations, which are used to verify the calculated values and conditions in the electroporated sample. Last but one part discusses the mechanical design of the workplace. There are described all problems of the construction and commissioning of the product. The last section is dedicated to the workplace measurement and analysis of the measured results.
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EKG synchonization of electroporation pulses
Nohel, Michal ; Mézl, Martin (referee) ; Novotná, Veronika (advisor)
Bachelor thesis is focused on cells electroporation principle and its types. It further describes thermal and nonthermal ablation techniques with an emphasis on irreversible electroporation. It contains an overview of fields, where medical procedures in combination with ECG signal are used, and an overview of techniques and measurements of the electrocardiographic signal. Further, the sensitivity of the human body to electroporation pulses is discussed. During electroporation, short high-voltage pulses are applied. Ventricular fibrillation may occur if the electroporation pulse is applied to the vulnerable period of the heart rhythm. Synchronization of pulse application with heart activity of the patient is necessary for health and safety reasons in clinical practice. The practical part of the thesis is about a LabVIEW program for this kind of synchronization. The created program is tested and the obtained results achieved are evaluated in terms of practical use.
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Gene expression in chicken embryo: micromanipulation and visualization methods
Bendová, Michaela
The aim of this work was to obtain better insight into the principles of cell structures and organs in the chicken embryo development. To reach this goal special methods of micromanipulations and visualization in vitro, ex vivo, ex ovo and in ovo were implemented and adjusted. These methods were used to study gene expression in neural crest development and eye development. In the course of long term research in our laboratory we observed that oncoprotein v-Myb influences the development of the neural crest and has the capacity to change natural cell fate. We performed a series of experiments to investigate v-Myb protein influence on neural crest cells differentiation, especially melanocyte lineage development, and its influence on gene expression in the neural crest. Therefore we focused on Gremlin 2 (PRDC), the gene upregulated by v-Myb in the neural crest. The established procedure of electroporation in ovo was adjusted to transfect cells of the developing eye and used to study gene expression during lens induction. The results obtained from chicken embryo experiments endorsed the study performed on mouse embryos. Futhermore, the electroporation technique was slightly modified for manipulations of the neural retina in the developing eye in ovo. Thereafter, the retinas were processed ex vivo and...
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High-voltage method for measuring the specific conductivity of tissue
Uher, Jakub ; Novotná, Veronika (referee) ; Červinka, Dalibor (advisor)
This master´s thesis is dedicated to the ever-improving medical method - electroporation. This work is divided into six chapters. The first two chapters describe what electroporation entails and how it affects the tissue, how we can evaluate it and how much influence the change in tissue temperature has on measurements. The third chapter deals with the design of coaxial arrangement of electrodes - from the initial simulation to the physical product. In the fourth chapter, the measuring workplace is shown. The penultimate chapter is described and processed by individual experiments carried out on animal tissue. The last, sixth chapter summarizes the possible inaccuracies that arise in the measurement and therefore affect the results.
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Gene expression in chicken embryo: micromanipulation and visualization methods
Bendová, Michaela ; Dvořák, Michal (advisor) ; Hirsch, Ivan (referee) ; Krylov, Vladimír (referee)
The aim of this work was to obtain better insight into the principles of cell structures and organs in the chicken embryo development. To reach this goal special methods of micromanipulations and visualization in vitro, ex vivo, ex ovo and in ovo were implemented and adjusted. These methods were used to study gene expression in neural crest development and eye development. In the course of long term research in our laboratory we observed that oncoprotein v-Myb influences the development of the neural crest and has the capacity to change natural cell fate. We performed a series of experiments to investigate v-Myb protein influence on neural crest cells differentiation, especially melanocyte lineage development, and its influence on gene expression in the neural crest. Therefore we focused on Gremlin 2 (PRDC), the gene upregulated by v-Myb in the neural crest. The established procedure of electroporation in ovo was adjusted to transfect cells of the developing eye and used to study gene expression during lens induction. The results obtained from chicken embryo experiments endorsed the study performed on mouse embryos. Futhermore, the electroporation technique was slightly modified for manipulations of the neural retina in the developing eye in ovo. Thereafter, the retinas were processed ex vivo and...
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